人FCGR3A / CD16a蛋白, His Tag

Human Fc gamma RIIIA / CD16a (V176) Protein, His Tag (SPR & BLI & MALS verified)

分子别名（Synonym）

FCGR3A, CD16A, FCG3, FCGR3, IGFR3

表达区间及表达系统（Source）

Human CD16a (V176), His Tag (CD8-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1 (F176V)).

Predicted N-terminus: Gly 17

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.7 kDa. The protein migrates as 40-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介绍

CD16作为低亲和力Fc受体，包含FcγRIIIa（CD16a）和FcγRIIIb（CD16b）两种亚型。这些受体特异性结合IgG抗体的Fc片段，由两个高度同源基因以细胞类型特异性方式编码，主要表达于自然杀伤细胞（NK细胞）、中性粒细胞、单核细胞及巨噬细胞表面。其中CD16a是跨膜糖蛋白（中等亲和力），分布于NK细胞、巨噬细胞、T细胞亚群、未成熟胸腺细胞和胎盘滋养层细胞；而CD16b为GPI锚定受体（低亲和力），仅表达于中性粒细胞和嗜酸性粒细胞。

CD16a在免疫应答中发挥多重作用：介导吞噬过程、调控酶类及炎症介质分泌、执行抗体依赖性细胞毒作用（ADCC）并参与免疫复合物清除。该受体表达异常或突变可导致反复病毒感染易感性增加，与系统性红斑狼疮（SLE）发病风险显著相关，并可能引发新生儿同种免疫性中性粒细胞减少症。