人TNF-alpha蛋白, His Tag

Human TNF-alpha Protein, His Tag, active trimer (MALS verified)

分子别名（Synonym）

DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα

表达区间及表达系统（Source）

Human TNF-alpha Protein, His Tag (TNA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).

Predicted N-terminus: Val 77

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 18.3 kDa. The protein migrates as 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

肿瘤坏死因子α（TNFα）是一种主要由单核细胞和巨噬细胞产生的细胞因子，存在于组织中的滑膜细胞和巨噬细胞内。其核心功能是调节免疫细胞活性：既能诱导细胞凋亡和炎症反应，亦可抑制肿瘤发生与病毒复制。TNFα分泌失调与多种人类疾病相关，包括重度抑郁症、阿尔茨海默病及恶性肿瘤。

该因子的重组形式作为免疫刺激剂应用于临床，国际非专利药品名（INN）为他索纳明（tasonermin）。在恶性肿瘤病理状态下，TNFα可发生异位分泌，其引发继发性高钙血症的作用机制及关联癌症类型与甲状旁腺素高度相似。