人IL-2RB & IL-2RG蛋白, Fc, Avitag

Biotinylated Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc,Avitag™&Fc,Avitag™ (MALS verified)

分子别名（Synonym）

IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG

表达区间及表达系统（Source）

Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag (ILG-H82F3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG) (Accession # P14784-1 (IL-2RB) & P31785-1 (IL-2RG)).

Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)

蛋白结构（Molecular Characterization）

Biotinylated Human IL-2RB&IL-2RG Heterodimer Protein, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 52.8 kDa (IL-2RB) and 55.3 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein migrates as 60-66 kDa and 80-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素-2受体β亚基和白细胞介素-2受体γ亚基均是白细胞介素-2的受体组成部分，它们作为多种白细胞受体共有的亚基单元。在白细胞介素刺激下，该亚基与SHB蛋白发生相互作用。可能通过与IL15RA协同作用，参与IL15对中性粒细胞吞噬功能的激活。此β亚基不仅参与受体介导的内吞作用，还转导IL2的促有丝分裂信号。

IL2R存在三种不同构型：高亲和力二聚体、中等亲和力单体（β亚基）及低亲和力单体（α亚基）。其中高亲和力与中等亲和力形式均需与γ亚基结合方可形成功能复合体。