人Insulin R蛋白, His Tag

Human Insulin R / CD220 (28-944) Protein, His Tag (MALS & SPR verified)

分子别名（Synonym）

INSR, Insulin receptor, IR, CD220

表达区间及表达系统（Source）

Human Insulin R (28-944), His Tag (INR-H52Ha) is expressed from human 293 cells (HEK293). It contains AA His 28 - Lys 944 (Accession # P06213-2).

Predicted N-terminus: His 28

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. The mature form of Insulin R a disulfide-linked heterotetramer composed of 2 alpha and 2 beta chains. Each α and β chain (partial fragment) has a calculated MW of 83.6 kDa and 22.8 kDa. The protein migrates as 120-130 kDa and 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

胰岛素受体（INSR）亦称CD抗原CD220，可被切割形成以下两条链：胰岛素受体α亚基和胰岛素受体β亚基。该受体是由2条α链和2条β链通过二硫键连接形成的四聚体。α链携带胰岛素结合区域，β链则具有激酶结构域。INSR可与IGF1R形成杂合受体，该杂合体由1条INSRα链、1条INSRβ链与1条IGF1Rα链、1条IGF1Rβ链共同构成四聚体。除结合胰岛素外，胰岛素受体还能结合胰岛素样生长因子（IGFI和IGFII）。INSR的短亚型对IGFII具有更高结合亲和力；当与IGF1R形成杂合受体时，INSR可结合IGF1。