ROR1蛋白, His Tag (MALS verified)

Human / Cynomolgus / Rhesus macaque ROR1 Protein, His Tag (MALS verified)

分子别名（Synonym）

ROR1,NTRKR1

表达区间及表达系统（Source）

Human / Cynomolgus / Rhesus macaque ROR1, His Tag (RO1-H522y) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403 (Accession # Q01973-1). In the region Gln 30 - Glu 403, the AA sequence of Human, Cynomolgus and Rhesus macaque ROR1 are homologus.

Predicted N-terminus: Gln 30

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 43.9 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 30 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介绍

酪氨酸蛋白激酶跨膜受体ROR1（亦称神经营养酪氨酸激酶受体相关蛋白1，NTRKR1）属于蛋白激酶超家族、酪氨酸蛋白激酶家族及ROR亚家族成员。该受体包含1个FZ（卷曲）结构域、1个Ig样C2型结构域、1个Kringle结构域和1个蛋白激酶结构域。ROR1在胚胎发育早期高表达，至第16天左右表达水平急剧下降，在成年组织中仅维持极低表达水平。其短亚型（Isoform Short）在胎儿及成人中枢神经系统以及多种人类癌症（包括源自中枢神经系统或周围神经系统神经外胚层的肿瘤）中显著表达。ROR1可通过与ε型酪蛋白激酶1（CK1ε）相互作用，激活磷酸肌醇3-激酶介导的AKT磷酸化及cAMP应答元件结合蛋白（CREB）信号通路，从而促进肿瘤细胞生长。