人CD2 / SRBC蛋白, His Tag

Human CD2 / SRBC Protein, His Tag (MALS verified)

分子别名（Synonym）

CD2,SRBC,LFA-2,T11

表达区间及表达系统（Source）

Human CD2, His Tag (CD2-H5226) is expressed from human 293 cells (HEK293). It contains AA Lys 25 - Asp 209 (Accession # AAH33583).

Predicted N-terminus: Lys 25

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.3 kDa. The protein migrates as 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

T细胞表面抗原CD2（亦称红细胞受体、LFA-2、LFA-3受体、玫瑰花结受体、T细胞表面抗原T11/Leu-5及SRBC）是一种表达于T细胞和自然杀伤（NK）细胞表面的单次跨膜I型膜蛋白。该蛋白属于免疫球蛋白超家族成员，包含一个Ig样C2型结构域和一个Ig样V型结构域。

CD2/SRBC可与其他细胞表面表达的黏附分子相互作用：在人类中主要与淋巴细胞功能相关抗原-3（LFA-3/CD58）结合，在啮齿类动物中则与CD48结合。除介导细胞黏附外，CD2还作为T细胞和NK细胞的共刺激分子发挥作用。作为T细胞和NK细胞的特异性标志物，CD2可通过免疫组织化学法用于组织切片中这类细胞的鉴定。