人M-CSF / CSF-1蛋白, premium grade

Human M-CSF / CSF-1 Protein, premium grade

分子别名（Synonym）

M-CSF,CSF-1,Lanimostim

表达区间及表达系统（Source）

Human M-CSF Protein, premium grade (MCF-H5218) is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1).

Predicted N-terminus: Glu 33

该产品在严格的质量控制体系下生产，包含无菌检测和内毒素检测等全面测试项目。产品性能经过精心验证，并在临床前研究阶段针对细胞培养及其他应用场景进行了相容性测试。

GMP-MCFH24是MCF-H5218的GMP版本，两种蛋白质的性能指标完全一致，可确保终端用户从早期临床前研究到后期临床试验阶段的无缝转换。

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 25.1 kDa. The protein migrates as 39 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method / rFC method.

宿主蛋白残留（Host Cell Protein）

<0.5 ng/µg of protein tested by ELISA.

宿主核酸残留（Host Cell DNA）

<0.02 ng/μg of protein tested by qPCR.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

集落刺激因子1（CSF1），亦称巨噬细胞集落刺激因子（M-CSF），是一种可影响造血干细胞分化为巨噬细胞或其他相关细胞类型的分泌型细胞因子。真核细胞也会产生M-CSF以抵抗细胞间病毒感染。该因子是三种经实验验证的集落刺激因子之一。M-CSF通过与集落刺激因子1受体结合发挥作用，并被证实可与PIK3R2发生相互作用。

作为造血生长因子，M-CSF（或称CSF-1）参与单核细胞、巨噬细胞及骨髓祖细胞的增殖、分化和存活过程。血管壁局部产生的M-CSF会促进动脉粥样硬化的发生与发展。