人APOE / Apolipoprotein E蛋白, His Tag

Human Apolipoprotein E / APOE3 Protein, His Tag

分子别名（Synonym）

Apolipoprotein E,APOE,Apo-E

表达区间及表达系统（Source）

Human APOE3, His Tag (APE-H5246) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - His 317 (Accession # P02649-1).

Predicted N-terminus: Lys 19

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.1 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

无菌（Sterility）

Negative

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

载脂蛋白E（APOE）是一种与脂质颗粒结合的载脂蛋白，其主要功能是通过血浆和组织液介导脂蛋白在器官间的脂质转运。APOE作为血浆脂蛋白的核心组分，参与脂蛋白的生成、转化与清除过程。载脂蛋白属于两亲性分子，既能与脂蛋白颗粒核心的脂质相互作用，又可适应血浆的水相环境。因此，APOE可与乳糜微粒、乳糜微粒残余物、极低密度脂蛋白（VLDL）及中间密度脂蛋白（IDL）结合，但对高密度脂蛋白（HDL）表现出优先结合特性。此外，APOE能与多种细胞受体结合，包括低密度脂蛋白受体（LDLR）、低密度脂蛋白受体相关蛋白（LRP1、LRP2、LRP8）以及极低密度脂蛋白受体（VLDLR），这些受体介导了含APOE脂蛋白颗粒的细胞摄取过程。