人整合素Integrin α4β1蛋白, His Tag&Tag Free

Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein, His Tag&Tag Free

分子别名（Synonym）

Integrin alpha 4 beta 1, ITGA4&ITGB1

表达区间及表达系统（Source）

Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).

Predicted N-terminus: Tyr 34 & Ser 592 (ITGA4) & Gln 21 (ITGB1)

蛋白结构（Molecular Characterization）

Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 111.5 kDa (ITGA4) and 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The ITGA4 subunit is composed of a heavy chain (Tyr 34 - Arg 591, calculated MW 61.1 kDa) and a light chain (Ser 592 - Thr 977, calculated MW 50.4 kDa). Consequently ITGA4 migrates as 60-75 kDa, and ITGB1 as 110-140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker respectively under non-reducing (NR) condition due to cleavage and glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

整合素是一类跨膜蛋白，可介导相邻细胞间黏附分子和/或细胞外基质（ECM）的相互作用。其在多种生物学过程中发挥重要作用，包括发育和伤口愈合过程中的细胞迁移、细胞分化以及细胞凋亡。整合素的活性还能调节肿瘤细胞的转移和侵袭潜能。整合素α4β1（Alpha-4/beta-1）是纤连蛋白的受体，同时也可作为VCAM1的受体并识别VCAM1中的Q-I-D-S序列。