小鼠FAP蛋白, His Tag, active dimer

Mouse FAP Protein, His Tag, active dimer (MALS verified)

分子别名（Synonym）

FAP,FAPalpha,SIMP,Seprase,APCE

表达区间及表达系统（Source）

Mouse FAP, His Tag (FAP-M52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 761 (Accession # P97321-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 87.1 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

FAP（亦称seprase）是一种II型跨膜丝氨酸蛋白酶。其质膜形式与可溶形式均具有脯氨酸后切割内肽酶活性，尤其倾向于识别Ala/Ser-Gly-Pro-Ser/Asn/Ala共识序列。该酶还能降解明胶和热变性I型胶原蛋白，同时具备二肽基肽酶活性，优先作用于Ala-Pro、Ile-Pro、Gly-Pro、Arg-Pro及Pro-Pro等二肽序列。质膜形式的FAP通过与DPP4、PLAUR或整联蛋白结合，参与细胞外基质（ECM）的周细胞蛋白水解过程，从而促进细胞在ECM中的粘附、迁移和侵袭。该酶通过降解蛋白聚糖brevican促进胶质瘤细胞在脑实质中的侵袭。在黑色素细胞中，FAP通过以不依赖丝氨酸蛋白酶活性的方式调控细胞增殖与存活，发挥肿瘤抑制因子作用。