Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, His Tag&Tag Free (MALS verified)
分子别名（Synonym）
Integrin alpha V beta 1,ITGAV&ITGB1
表达区间及表达系统（Source）
Human ITGAV&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1) (Accession # NP_002201.1 (ITGAV) & NP_002202.2 (ITGB1)).
Predicted N-terminus: Phe 31 (ITGAV) & Gln 21 (ITGB1)
蛋白结构（Molecular Characterization）
Human ITGAV&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 112.9 kDa (ITGAV) and 83.7 kDa (ITGB1). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 125-150 kDa (ITGAV) and 95-110 kDa (ITGB1) respectively due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
整合素α-5/β-1是纤维蛋白原（fibrinogen）的受体*。整合素α-1/β-1、α-2/β-1、α-6/β-1和α-7/β-1是层粘连蛋白（laminin）的受体。整合素α-4/β-1是VCAM1的受体，能识别VCAM1中的Q-I-D-S序列。整合素α-9/β-1是VCAM1、细胞趋触蛋白（cytotactin）和骨桥蛋白（osteopontin）的受体，能识别细胞趋触蛋白中的A-E-I-D-G-I-E-L序列。整合素α-V/β-1也是玻连蛋白（vitronectin）的受体。β-1整合素亚族能识别多种配体中普遍存在的R-G-D序列。亚型2会干扰亚型1的功能，从而对细胞粘附和迁移产生显性负效应（体外实验证实）。当与α-7/β-1整合素结合时，可调控细胞粘附与层粘连蛋白基质沉积。
