食蟹猴OX40L / OX40 Ligand蛋白, His Tag

Cynomolgus / Rhesus macaque OX40 Ligand Protein, His Tag, active trimer (MALS verified) 

分子别名（Synonym）

OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L

表达区间及表达系统（Source）

Cynomolgus / Rhesus macaque OX40 Ligand Protein, His Tag (OXL-C5243) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # A0A1D5QQH7 ).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 49.3 kDa. The protein migrates as 65-80 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

肿瘤坏死因子配体超家族成员4（TNFSF4）亦称糖蛋白Gp34、OX40配体（OX40L）、TAX转录激活糖蛋白1及CD252，属于肿瘤坏死因子家族。该蛋白是CD134的配体，表达于树突细胞亚型DC2等细胞表面，可放大Th2细胞分化效应。TNFSF4与其受体的相互作用参与多种自身免疫性疾病和炎症性疾病的发病机制，包括系统性红斑狼疮（SLE）、颈动脉疾病及癌症。此外，与其他TNF超家族成员类似，膜结合型OX40配体（TNFSF4）以同源三聚体形式存在。人类TNFSF4蛋白与小鼠对应蛋白具有46%的氨基酸序列同源性。