人CD27 Ligand / CD70蛋白, His, Flag Tag

Human CD27 Ligand / CD70 Protein, His,Flag Tag, active trimer (MALS verified)

分子别名（Synonym）

CD70,CD27LG,TNFSF7,TNFSF7G,CD27L

表达区间及表达系统（Source）

Human CD27 Ligand Protein, His,Flag Tag (CDL-H52Da) is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1) trimer Design.

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 49.3 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

分化簇70（CD70）亦称为CD27配体（CD27L/CD27LG）或肿瘤坏死因子超家族成员7（TNFSF7/TNFSF7G），是一种属于TNF超家族（TNFSF）的II型跨膜糖蛋白，作为表面抗原表达于活化的T淋巴细胞、B淋巴细胞及成熟树突状细胞表面。CD70通过与受体CD27结合，可诱导T细胞的启动、效应功能发挥、分化及记忆形成，从而参与T细胞活化、共刺激T细胞增殖以及细胞毒性T细胞生成等生物学过程。T细胞表面的CD70能提供T细胞增殖、克隆扩增及促进效应T细胞形成所需的共刺激信号。研究表明，小鼠B细胞表面的CD70可抑制活化B细胞向浆细胞的终末分化，并增强记忆B细胞应答的定向分化。在自然杀伤细胞（NK细胞）中，CD70能诱导细胞增殖并促进γ干扰素（IFNγ）的产生。