间皮素Mesothelin / MSLN蛋白, His Tag, premium grade

Human Mesothelin / MSLN (296-580) Protein, His Tag, premium grade

分子别名（Synonym）

MSLN,Mesothelin,MPF

表达区间及表达系统（Source）

Human Mesothelin (296-580), His Tag, premium grade (MSN-H522a) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272).

Predicted N-terminus: Glu 296

本产品在生产过程中遵循严格的质量控制体系，涵盖无菌及内毒素检测等全方位测试。我们会对产品性能进行严谨验证，确保其适用于细胞培养及临床前研究阶段的其他应用。当您的研究进入后期临床阶段时，我们还可提供定制化GMP蛋白质服务。我们将根据您的需求，共同开发符合细胞疗法生产中原材料与辅助材料使用要求的GMP级别产品。

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 33.0 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method / rFC method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

间皮素（MSLN）亦称CAK1抗原、前巨核细胞强化因子原，属于间皮素家族蛋白。该蛋白可通过弗林蛋白酶样转化酶发生蛋白水解裂解，形成两个活性片段：巨核细胞强化因子（MPF）和间皮素裂解形式。MPF与间皮素裂解形式均具有N-糖基化修饰特性。间皮素/MSLN能与MUC16发生相互作用，其膜锚定形式可能在细胞粘附过程中发挥作用，而MPF在体外实验中显示可增强巨核细胞集落形成能力。