人IL-2 R gamma / CD132蛋白, His Tag

Human IL-2 R gamma / CD132 Protein, His Tag (MALS verified)

分子别名（Synonym）

IL2RG,CD132,CIDX,IMD4,P64,SCIDX,SCIDX1,gammaC

表达区间及表达系统（Source）

Human IL-2 R gamma, His Tag (ILA-H52H5) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Asn 254 (Accession # P31785-1).

Predicted N-terminus: Leu 23

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 29.3 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素2受体（IL-2R）是一种异源三聚体蛋白，可结合并响应细胞因子IL-2。该受体由α、β和γ三条非共价结合的多肽链构成。其中α链与β链参与IL-2的结合，而细胞因子结合后的信号转导则由γ链与β亚基共同完成。在与IL-2结合前，IL-2R的α链即可与β链发生结合。γ链单独对IL-2的亲和力极弱，但当配体与α/β异二聚体结合后，γ链会被招募至复合体中，形成高度稳定的大分子四级配体/受体复合物。

白细胞介素2受体γ亚基（IL2RG）又称细胞因子受体共同γ链、CD抗原CD132、gammaC、p64，属于I型细胞因子受体家族中的第5亚族。该蛋白表达于骨髓未成熟造血细胞表面，其基因缺陷会导致X连锁严重联合免疫缺陷症（表现为T细胞缺乏、B细胞正常、NK细胞缺乏的XSCID）。