人GLP1R蛋白, His, Avitag

Biotinylated Human GLP1R Protein, His,Avitag™ (HPLC verified)

分子别名（Synonym）

GLP-1-R, GLP-1R, glucagon like peptide 1 receptor

表达区间及表达系统（Source）

Biotinylated Human GLP1R, His,Avitag (GLR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Glu 139 (Accession # P43220-1).

Predicted N-terminus: Ala 21

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 17.3 kDa. The protein migrates as 30-34 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

该基因编码一种7次跨膜蛋白，其功能是作为胰高血糖素样肽-1（GLP-1）激素的受体，可刺激葡萄糖诱导的胰岛素分泌。该受体在细胞表面发挥作用，遇到GLP-1及其类似物时会发生内化，并在介导胰岛素分泌的信号传导级联反应中起重要作用。在动物模型中，该受体还表现出神经保护作用。该基因的多态性与糖尿病发病相关，其编码的蛋白是治疗2型糖尿病和中风的重要药物靶点。