人LRRC15 / LIB蛋白, His Tag

Human LRRC15 / LIB Protein, His Tag (MALS verified)

分子别名（Synonym）

Leucine-rich repeat-containing protein 15,LRRC15,LIB,hLib

表达区间及表达系统（Source）

Human LRRC15, His Tag (LR5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Tyr 22 - Gly 538 (Accession # Q8TF66-1).

Predicted N-terminus: Tyr 22

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 59.8 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

LRRC15（富含亮氨酸重复序列蛋白15）亦称为LIB和hLib。该蛋白在多种实体瘤中高表达，见于众多实体瘤（如乳腺癌、头颈癌、肺癌、胰腺癌）的基质成纤维细胞表面，并直接表达于部分间充质来源的癌细胞（如肉瘤、黑色素瘤、胶质母细胞瘤）上。TGFβ可诱导活化成纤维细胞（αSMA+标志）和间充质干细胞表达LRRC15。这些发现共同表明，LRRC15可作为新型癌症相关成纤维细胞（CAF）和间充质标志物，为治疗LRRC15阳性间质纤维化或间充质来源的癌症提供了潜在治疗靶点。ABBV-085是一种靶向LRRC15的抗体药物偶联物（ADC），含单甲基澳瑞他汀E（MMAE）有效载荷。临床前研究显示，作为单药或与标准疗法联用时，该药物对LRRC15基质阳性/癌细胞阴性模型及LRRC15癌细胞阳性模型均具有显著抗肿瘤活性。