人CD40 / TNFRSF5蛋白, His Tag

Human CD40 / TNFRSF5 Protein, His Tag (MALS verified)

分子别名（Synonym）

CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50

表达区间及表达系统（Source）

Human CD40, His Tag (CD0-H5228) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1).

Predicted N-terminus: Glu 21

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 21.1 kDa. The protein migrates as 29-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

CD40（亦称为TNFRSF5、Bp50、CDW40、MGC9013、TNFRSF5及p50）是肿瘤坏死因子受体超家族成员，该家族均为单次跨膜糖蛋白。CD40在介导多种免疫及炎症反应中发挥关键作用，包括T细胞依赖性免疫球蛋白类别转换、记忆性B细胞发育以及生发中心形成。作为抗原呈递细胞上的共刺激蛋白，CD40对其活化至关重要。TH细胞表面的CD154（CD40L）与CD40结合后，可激活抗原呈递细胞并诱导多种下游效应。

CD40胞外结构域包含4个富含半胱氨酸的重复序列，表达于B细胞、树突细胞、巨噬细胞、内皮细胞及多种肿瘤细胞系中。这种富含半胱氨酸的重复区域是TNF超家族受体的典型特征。CD40与其配体CD40L结合后可引起分子聚集，进而与胞质组分相互作用启动信号通路。早期对CD40-CD40L系统的研究揭示了其在体液免疫中的作用。CD40缺陷会导致3型高IgM免疫缺陷症（HIGM3），这是一种常染色体隐性遗传病，特征为B细胞无法进行同种型转换、不能产生抗体特异性免疫应答以及生发中心形成障碍。