人IL-17F蛋白, His Tag (MALS verified)

Human IL-17F Protein, His Tag (MALS verified)

分子别名（Synonym）

IL-17F, Interleukin-17F, Cytokine ML-1

表达区间及表达系统（Source）

Human IL-17F, His Tag (ILF-H5244) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 16.8 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a dimer.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素17F（IL17F）亦称白细胞介素24（IL24）或细胞因子ML-1，是一种通过二硫键连接的同源二聚体分泌蛋白，属于IL-17家族。人类和小鼠的IL-17家族至少包含六个成员：IL-17A、IL-17B、IL-17C、IL-17D、IL-17E（又称IL-25）及IL-17F（又称IL-24）。该家族细胞因子具有结构同源性，其C端区域高度保守，但N端区域各不相同且生物学功能各异。

IL-17F/IL-24表达于活化状态的CD4+ T细胞及活化单核细胞（静息状态下不表达）。它能刺激IL-6、IL-8和粒细胞集落刺激因子等其他细胞因子的产生，并调节软骨基质代谢。此外，IL-17F/IL-24可促进外周血单核细胞（PBMC）和T细胞增殖，并抑制血管生成。IL17F基因缺陷是导致6型家族性念珠菌病（CANDF6）的病因。