人EGFR蛋白, Fc Tag

Human EGF R Protein, Fc Tag (MALS verified)

分子别名（Synonym）

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

表达区间及表达系统（Source）

Human EGF R, Fc Tag (EGR-H5252) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).

Predicted N-terminus: Leu 25

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 94.7 kDa. The protein migrates as 110-140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

表皮生长因子受体（EGFR，又称ErbB-1，人类中称为HER1）是细胞表面受体，可结合细胞外蛋白配体表皮生长因子家族（EGF家族）成员。该受体属于ErbB受体家族——一个由四种密切相关的受体酪氨酸激酶组成的亚家族，包括EGFR（ErbB-1）、HER2/c-neu（ErbB-2）、Her3（ErbB-3）及Her4（ErbB-4）。影响EGFR表达或活性的突变可能诱发癌症。