人EpCAM / TROP1蛋白, His Tag

Human EpCAM / TROP1 Protein, His Tag

分子别名（Synonym）

EPCAM,TACSTD1,TROP1,CD326,DIAR5,EGP2,EGP314,EGP40,ESA,GA733-2,HNPCC8,HNPCC-8,KS1,4,KSA,M4S1,MIC18,MK1

表达区间及表达系统（Source）

Human EpCAM, His Tag (EPM-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1).

Predicted N-terminus: Gln 24

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 28.2 kDa. The protein migrates as 31-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

EpCAM亦称为CO171A、EGP、EGP40、GA7332、KSA、M4S1、MIC18、MK1、TROP1及hEGP2，是一种泛上皮分化抗原，在几乎所有上皮源性肿瘤中均以17-1A（单克隆抗体）抗原形式表达。其构成性功能正在被逐步阐明：该分子与钙黏蛋白-连环蛋白通路存在复杂关联，进而影响负责细胞内信号传导与极性维持的核心WNT通路。研究表明，上皮细胞粘附分子（Ep-CAM）在大多数上皮源性恶性肿瘤中均有表达，已被确认为肿瘤标志物及分子靶向治疗的候选靶点。

有报道显示，Ep-CAM与N-钙黏蛋白的交叉信号传导通过Pi3K途径发挥作用，导致上皮细胞中钙黏蛋白粘附复合物的功能丧失。近年来，上皮细胞粘附分子（Ep-CAM）作为乳腺癌预后因子日益受到关注。