猴CD19蛋白, His Tag

Cynomolgus / Rhesus macaque CD19 (20-292) Protein, His Tag

分子别名（Synonym）

CD19,B4,CVID3,MGC12802

表达区间及表达系统（Source）

Cynomolgus / Rhesus macaque CD19 (20-292), His Tag (CD9-C5221) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 292 (Accession # F7F486-1).

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 32.1 kDa. The protein migrates as 45-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

B淋巴细胞抗原CD19（亦称分化簇19，简称CD19）是一种单次跨膜I型蛋白，包含两个免疫球蛋白样C2型结构域。该蛋白表达于滤泡树突细胞和B细胞表面，从发育过程中最早可识别的B系细胞到B母细胞阶段持续存在，但在成熟为浆细胞时逐渐消失。

CD19主要作为B细胞共受体，与CD21和CD81协同作用。活化后其胞质尾部发生磷酸化，导致Src家族激酶结合并募集PI-3激酶。与T细胞类似，B淋巴细胞表面多个分子共同构成抗原受体复合物：其中（几乎）B细胞特异性的CD19磷酸糖蛋白是关键组分，其余包括CD21与CD81。这些表面免疫球蛋白（sIg）关联分子共同促进信号转导。

在活体B细胞中，模拟外源抗原的抗免疫球蛋白抗体会引发CD19与sIg结合并随之内化。反向过程尚未被证实，表明该受体复合物的形成是由抗原诱导的——这一分子关联已通过化学研究得到验证。CD19基因突变会导致以抗体产生减少为特征的严重免疫缺陷综合征。研究证实CD19可与CD81、CD82、补体受体2及VAV2发生相互作用。