人CD117 / c-kit蛋白, His, Avitag

Biotinylated Human CD117 / c-kit Protein, His,Avitag (MALS verified)

分子别名（Synonym）

CD117,SCFR,c-Kit,KIT

表达区间及表达系统（Source）

Biotinylated Human CD117, His,Avitag (CD7-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Thr 516 (Accession # P10721-2).

Predicted N-terminus: Gln 26

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 58.8 kDa. The protein migrates as 70-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

肥大/干细胞生长因子受体Kit（c-Kit）是蛋白激酶超家族、Tyr蛋白激酶家族及CSF-1/PDGF受体亚家族成员，亦被称为花斑性状蛋白（PBT）、p145 c-kit、酪氨酸蛋白激酶Kit或CD117。该受体包含五个Ig样C2型（免疫球蛋白样）结构域及一个蛋白激酶结构域。

CD117作为细胞因子KITLG/SCF的细胞表面受体，在细胞存活与增殖调控、造血作用、干细胞维持、配子发生、肥大细胞发育迁移与功能以及黑色素生成中起关键作用。结合KITLG/SCF后，CD117可激活多条信号通路。此外，活化后的KIT能促进蛋白磷酸酶PTPN6/SHP-1与PTPRU以及转录因子STAT1、STAT3、STAT5A和STAT5B的磷酸化。