人PD-1 / PDCD1蛋白, Fc, Avitag

Biotinylated Human PD-1 / PDCD1 Protein, Fc,Avitag™ (MALS verified)

分子别名（Synonym）

PDCD1, PD1, CD279, SLEB2

表达区间及表达系统（Source）

Biotinylated Human PD-1, Fc,Avitag (PD1-H82F1) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).

Predicted N-terminus: Leu 25

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 44.4 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

程序性细胞死亡蛋白1（PD-1）亦称CD279或PDCD1，是一种I型膜蛋白，属于扩展型CD28/CTLA-4 T细胞调节家族成员。该蛋白表达于活化的T细胞、B细胞、巨噬细胞、髓样细胞及部分胸腺细胞表面。PD-1拥有两个B7家族配体PD-L1和PD-L2：PD-L1广泛表达于几乎所有经IFN-γ处理的鼠源肿瘤细胞系（包括PA1骨髓瘤、P815肥大细胞瘤及B16黑色素瘤）；PD-L2表达范围较窄，主要存在于树突状细胞和少数肿瘤细胞系。

PD-1通过抑制PI3K/AKT通路的激活与转导，进而抑制T细胞增殖及IL-1、IL-4、IL-10和IFN-γ等相关细胞因子的产生。此外，PD-1共接可通过关键信号转导分子的去磷酸化作用，抑制BCR介导的信号传导。体外实验表明，使用PD-L1-Ig处理抗CD3刺激的T细胞后，T细胞增殖能力和IFN-γ分泌水平均显著降低。目前针对PD-1的单克隆抗体药物正在研发中，通过增强免疫系统功能来实现癌症治疗目的。