猴TL1A / TNFSF15蛋白, His Tag (MALS verified)

Cynomolgus/Rhesus macaque TL1A / TNFSF15 Protein, His Tag (MALS verified)

分子别名（Synonym）

TL1A, VEGI, TNFSF15

表达区间及表达系统（Source）

Cynomolgus/Rhesus macaque TL1A / TNFSF15, His Tag (TLA-C5241) is expressed from human 293 cells (HEK293). It contains AA Leu 72 - Leu 251 (Accession # F6S8F9-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 22.3 kDa. The protein migrates as 27-28 kDa and 29-31 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a trimer.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

肿瘤坏死因子样细胞因子1A（TL1A）及其受体——死亡受体3（DR3）与诱骗受体3（DcR3）分别属于肿瘤坏死因子超家族和肿瘤坏死因子受体超家族蛋白。抗原呈递细胞源性TL1A与淋巴细胞DR3的结合可为活化淋巴细胞提供共刺激信号。DR3信号传导不仅影响效应淋巴细胞的增殖活性及细胞因子产生，还关键性地调节调节性T细胞的发育与抑制功能。而DcR3则通过减弱T细胞活化、下调促炎细胞因子分泌，从而限制TL1A/DR3复合物的功能。TL1A与DR3、DcR3共同构成一个主动参与免疫应答调控的细胞因子系统。