人Fc gamma RIIIB / CD16b (NA2)蛋白, His Tag

Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI & MALS verified)

分子别名（Synonym）

Fc gamma RIIIB,CD16b (NA2),FCGR3B,CD16B,FCG3B,FCGR3,FCG3,IGFR3

表达区间及表达系统（Source）

Human CD16b (NA2), His Tag (CDB-H5222) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Ser 200 (Accession # O75015-1). The NA1 form of the CD16b differ with the NA2 form of CD16b in AA36, 65, 82, and 106. The NA1 form carries R36, N65, D82, and V106, while the NA2 form carries S36, S65, N82, and I106.

Predicted N-terminus: Gly 17

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.7 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介绍

CD16是一种低亲和力Fc受体，已被鉴定为FcγRIIIa（CD16a）和FcγRIIIb（CD16b）两种亚型。这些受体可与IgG抗体的Fc段结合。CD16由两个高度同源基因以细胞类型特异性方式编码，表达于自然杀伤细胞、中性多形核白细胞、单核细胞及巨噬细胞表面。

CD16B（亦称FCGR3B和FCG3B）特异性表达于多形核白细胞（中性粒细胞）和受刺激的嗜酸性粒细胞。该分子是免疫球蛋白γ段Fc区的低亲和力受体，既能结合复合或聚合形式的IgG，也能结合单体IgG。与III-A亚型相反，FCG3B不能介导抗体依赖性细胞毒作用及吞噬作用。CD16B可能作为外周循环中免疫复合物的捕获装置，且不会激活中性粒细胞。