人CD8 alpha / CD8A蛋白, His, Avitag

Biotinylated Human CD8 alpha / CD8A Protein, His,Avitag™

分子别名（Synonym）

CD8A,CD8,Leu2,MAL,p32

表达区间及表达系统（Source）

Biotinylated Human CD8 alpha, His,Avitag (CDA-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (Accession # P01732-1).

Predicted N-terminus: Ser 22

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 21.2 kDa. The protein migrates as 25-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

整合膜糖蛋白，在免疫应答中发挥关键作用，对外源性和内源性侵害具有多重防御功能。该蛋白可同时与T细胞受体（TCR）及抗原呈递细胞（APCs）提供的MHC I类蛋白相互作用，进而将Src激酶LCK招募至TCR-CD3复合物周边。在NK细胞中，CD8A同源二聚体在细胞表面的存在为多重靶细胞的结合与裂解提供了生存机制。此外，CD8A同源二聚体分子还能促进活化淋巴细胞的存活并分化为记忆性CD8 T细胞。