人IL-23A & IL-12B蛋白, premium grade

Human IL-23 alpha & IL-12 beta Heterodimer Protein, premium grade

分子别名（Synonym）

IL-23 alpha & IL-12 beta

表达区间及表达系统（Source）

Human IL-23A&IL-12B Heterodimer Protein, premium grade (ILB-H5219) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 20 - Pro 189 (IL-23A) (Accession # P29460-1 (IL-12B) & Q9NPF7-1 (IL-23A)).

Predicted N-terminus: Ile 23

该产品在我们严格的质量控制体系下生产，涵盖无菌检测和内毒素检测等全方位测试。产品性能经过精心验证，并针对细胞培养用途及临床前研究阶段的其他应用进行相容性测试。当研究需向后期临床阶段推进时，我们还提供定制化GMP蛋白质服务，可根据您的需求定制开发符合GMP级别的产品。我们将与您协同合作，根据您的要求定制开发符合细胞治疗产品生产所需原料与辅助材料标准的GMP级产品。

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 55.0 kDa. The protein migrates as 58 kDa±3 kDa under reducing (R) condition, and 58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素-23亚基α（IL-23 alpha）可与IL12B结合形成IL-23白细胞介素，这是一种在先天性与适应性免疫中发挥作用的异二聚体细胞因子。IL-23可能与IL-17共同构成外周组织对感染的急性应答机制。IL-23通过与由IL12RB1和IL23R组成的异二聚体受体复合物结合，激活Jak-Stat信号传导级联反应，刺激记忆T细胞（而非初始T细胞）并促进促炎细胞因子的产生。IL-23会诱导自身免疫性炎症，因此可能是自身免疫性炎症疾病的致病因子，并在肿瘤发生过程中发挥重要作用。