人CD38蛋白, His Tag

Human CD38 Protein, His Tag (MALS verified)

分子别名（Synonym）

CD38, T10, cADPr hydrolase 1

表达区间及表达系统（Source）

Human CD38, His Tag (CD8-H5224) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # NP_001766).

Predicted N-terminus: Val 43

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 30.7 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.2 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM MES, 100 mM NaCl, pH6.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

CD抗原CD38（又称ADP-核糖基环化酶1）属于ADP-核糖基环化酶家族，在胰腺、肝脏、肾脏、大脑、睾丸、卵巢、胎盘、恶性淋巴瘤和神经母细胞瘤中高表达。该蛋白是一种多功能外酶，可催化从NAD+合成环ADP-核糖（cADPR）并水解为ADP-核糖，这些反应产物对细胞内钙离子的调控至关重要。

CD38功能缺失与免疫应答缺陷、代谢紊乱和行为改变相关。其蛋白作为细胞活化标志物，与HIV感染、白血病、骨髓瘤、实体瘤、2型糖尿病及骨代谢密切相关，现已被用作白血病的预后标志物。