人BDNF / Abrineurin蛋白, premium grade

Human BDNF / Abrineurin Protein (HEK293), premium grade

分子别名（Synonym）

BDNF,ANON2,BULN2,Abrineurin

表达区间及表达系统（Source）

Human BDNF Protein (HEK293), premium grade (BDF-H5219) is expressed from human 293 cells (HEK293). It contains AA His 129 - Arg 247 (Accession # P23560-1).

Predicted N-terminus: His 129

本产品在严格的质量控制体系下生产，该体系涵盖无菌及内毒素检测等全套测试项目。其产品性能经过严谨验证，针对细胞培养应用及临床前阶段的其他用途均进行了兼容性测试。当您的研究需推进至后期临床试验阶段时，我们同步提供定制化GMP蛋白服务——根据您的需求，我们将提供全程协作，开发符合细胞治疗产品制造要求的GMP级蛋白制品，同时满足原辅材料使用规范。

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 13.5 kDa. The protein migrates as 13-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method / rFC method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

脑源性神经营养因子（BDNF）与神经生长因子（NGF）、神经营养因子3（NT-3）、神经营养因子4/5（NT-4/5）同属神经营养因子家族。这类因子对周围及中枢神经系统各类神经元的发育、存活及功能维持至关重要。神经营养因子通过两类细胞表面受体发挥作用：低亲和力P75受体与高亲和力酪氨酸激酶受体Trk家族。其中，NGF主要结合TrkA；BDNF及NT4/5特异性结合TrkB；NT-3则以TrkC为主要结合靶点（亦可较弱结合TrkA）。