PE-Labeled人CD19蛋白, His Tag

PE-Labeled Human CD19 (20-291) Protein, His Tag

分子别名（Synonym）

CD19, B4, CVID3, MGC12802

表达区间及表达系统（Source）

PE-Labeled Human CD19 (20-291), His Tag (CD9-HP2H5) is produced via conjugation of PE to Human CD19 (20-291), His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD19 (20-291), His Tag is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

偶联（Conjugate）

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

B淋巴细胞抗原CD19（亦称CD19或分化簇19）是一种单次跨膜I型膜蛋白，包含两个免疫球蛋白样C2型结构域。该蛋白表达于滤泡树突细胞及B细胞表面——事实上，从发育过程中最早可识别的B系细胞到B母细胞阶段持续存在，但在成熟为浆细胞时消失。CD19主要作为B细胞共受体，与CD21及CD81协同作用。激活状态下，其胞质尾区发生磷酸化，进而招募Src家族激酶及PI-3激酶。

与T细胞类似，B淋巴细胞表面多个分子共同构成抗原受体复合物。CD19（几乎为B细胞特异性表达的磷酸化糖蛋白）即为关键组分，其他成员包括CD21与CD81。这些表面免疫球蛋白（sIg）关联分子协同介导信号转导：活体B细胞中，模拟外源抗原的抗免疫球蛋白抗体会引发CD19与sIg结合并随之内化，而逆向过程尚未证实，表明该受体复合物的形成由抗原诱导（此分子互作机制已获化学研究证实）。

CD19基因突变可导致以抗体生成减少为特征的重度免疫缺陷综合征。经证实，CD19可与下列分子相互作用：CD81、CD82、补体受体2及VAV2。