APC-Labeled人CD19(20-291)蛋白, His Tag

APC-Labeled Human CD19 (20-291) Protein, His Tag (Site-specific conjugation)

分子别名（Synonym）

CD19,B4,CVID3,MGC12802

表达区间及表达系统（Source）

APC-Labeled Human CD19 (20-291), His Tag (CD9-HA2H5) is produced via conjugation of APC to Human CD19 (20-291), His Tag with a new generation site-specific technology under optimal conditions with a proprietary technology. Human CD19 (20-291), His Tag is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).

Predicted N-terminus: Pro 20

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 33.7 kDa.

偶联（Conjugate）

APC

Excitation Wavelength: 640 nm

Emission Wavelength: 661 nm

应用说明（Application）

Please note that this product is NOT compatible to streptavidin detection system.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

B淋巴细胞抗原CD19（亦称分化簇19/CD19）是一种单次跨膜I型膜蛋白，包含两个免疫球蛋白样C2型结构域。该蛋白表达于滤泡树突状细胞及B细胞表面，具体而言：从发育早期可识别的B系祖细胞直至B母细胞阶段持续存在，但在成熟为浆细胞时消失。CD19主要作为B细胞共受体，与CD21、CD81协同作用。其胞质尾区在激活后被磷酸化，进而招募Src家族激酶及PI-3激酶形成复合物。与T细胞类似，B淋巴细胞表面多个分子共同构成抗原受体复合物，其中（几乎）B细胞特异性的CD19磷酸糖蛋白是该复合物的核心组分，其他成员包括CD21与CD81。这些表面免疫球蛋白（sIg）关联分子协同促进信号转导：活体B细胞中，模拟外源抗原的抗免疫球蛋白抗体会引发CD19与sIg结合并随之内化。反向过程尚未证实，提示该受体复合物的形成具有抗原诱导特性（该分子相互作用已获生化研究确证）。CD19基因突变可导致抗体产生缺陷型重症免疫缺陷综合征。经证实，CD19可与下列分子相互作用：CD81、CD82、补体受体2及VAV2。