人IL2RA / CD25蛋白, His Tag

Human IL-2 R alpha / CD25 Protein, His Tag

分子别名（Synonym）

IL2RA,CD25,p55,IL2-RA,IL-2-RA

表达区间及表达系统（Source）

Human IL-2 R alpha, His Tag (ILA-H52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).

Predicted N-terminus: Glu 22

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 23.6 kDa. The protein migrates as 34-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素-2受体α亚基（IL2RA）又称IL-2Rα、TAC抗原、p55或CD25抗原，属I型跨膜糖蛋白。该分子表达于活化T细胞和调节性T细胞表面，可独立以低亲和力结合IL-2。当IL2RA与IL-2受体β链及γ链通过非共价键组装成配体诱导的高亲和力异源三聚体受体复合物后，可启动MAPK、JAK/STAT等细胞内信号通路。在树突细胞（DC）中，CD25传统被视为活化标志物——尽管小鼠与人类DC均能表达CD25，但因缺乏IL-2受体β链（该链为IL-2信号传导不可或缺的组分），此类细胞无法执行IL-2信号转导功能。