人IL-17A / CTLA-8蛋白, His, Avitag

Biotinylated Human IL-17A / CTLA-8 Protein, His,Avitag™

分子别名（Synonym）

IL-17A,Interleukin-17A,CTLA-8,IL-17

表达区间及表达系统（Source）

Biotinylated Human IL17A, His,Avitag (ILA-H82Q1) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (Accession # Q16552-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 17.8 kDa. The protein migrates as 18-21 kDa and 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素17A（IL-17A），亦称细胞毒性T淋巴细胞相关抗原8（CTLA8），是由活化T细胞分泌的促炎细胞因子。该因子可调控核因子κB（NF-κB）和丝裂原活化蛋白激酶的活性，并能刺激IL6及环氧合酶-2（PTGS2/COX-2）的表达，同时增强一氧化氮（NO）的生成。此外，IL-17A存在糖基化与非糖基化两种形式，其水平升高与类风湿关节炎、银屑病及多发性硬化症等多种慢性炎症性疾病密切相关。IL-17A通过与其受体IL-17RA结合，激活下游信号通路，从而诱导多种炎症介质的释放，包括细胞因子、趋化因子和黏附分子，进一步招募和激活免疫细胞，放大炎症反应。研究发现，IL-17A在宿主防御中亦发挥关键作用，尤其是在对抗胞外细菌和真菌感染方面。然而，其过度活化或失调亦会导致组织损伤和自身免疫病的发生。因此，靶向IL-17A或其信号通路的治疗策略已在多种炎症性疾病中展现出良好的临床前景。