人GDF-15 / MIC-1蛋白, His Tag

Human GDF-15 / MIC-1 Protein, His Tag

分子别名（Synonym）

GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1

表达区间及表达系统（Source）

Human GDF-15, His Tag (GD5-H5149) is expressed from E. coli cells. It contains AA Ala 197 - Ile 308 (Accession # Q99988-1).

Predicted N-terminus: Met

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 14.2 kDa. The protein migrates as 15 kDa under reducing (R) condition, and 27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 0.2 EU per μg by the LAL method / rFC method.

无菌（Sterility）

Negative

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 30% ACN, 0.085% TFA with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

生长分化因子15（GDF-15），亦称巨噬细胞抑制因子1（MIC-1）。在单核细胞中，MIC-1 mRNA的表达受到多种激活相关刺激因子的上调，包括白细胞介素1β、肿瘤坏死因子α（TNF-α）、白细胞介素2和巨噬细胞集落刺激因子，但干扰素γ或脂多糖（LPS）无此作用。该因子在正常及病理状态下均高表达于心肌细胞、脂肪细胞、巨噬细胞、内皮细胞和血管平滑肌细胞。组织损伤和炎症状态时GDF-15水平升高，且与心血管代谢风险相关。糖尿病患者的GDF-15水平升高与心肌肥厚、心力衰竭、动脉粥样硬化、内皮功能障碍、肥胖、胰岛素抵抗、糖尿病及慢性肾脏病等心血管疾病密切相关。GDF-15水平的升高与疾病进展及预后存在显著关联。