人FAP蛋白, His Tag, active dimer

Human FAP Protein, His Tag, active dimer (MALS verified)

分子别名（Synonym）

FAP, FAPalpha, SIMP, Seprase, APCE

表达区间及表达系统（Source）

Human FAP, His Tag (FAP-H5244) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 86.9 kDa. The protein migrates as 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

成纤维细胞活化蛋白（FAP/分离酶）是一种II型跨膜丝氨酸蛋白酶，其膜结合型与可溶型均具有脯氨酸后切割内肽酶活性，尤其偏好切割Ala/Ser-Gly-Pro-Ser/Asn/Ala共识序列；同时可降解明胶及热变性I型胶原，并展现二肽基肽酶活性（优先作用于Ala-Pro、Ile-Pro、Gly-Pro、Arg-Pro和Pro-Pro）。膜结合型FAP通过与DPP4、PLAUR或整合素结合，介导细胞外基质（ECM）的细胞周蛋白水解，进而促进细胞粘附、迁移及侵袭；在神经胶质瘤中通过降解蛋白聚糖短蛋白聚糖促进脑实质侵袭。值得注意的是，该蛋白在黑色素细胞中通过不依赖丝氨酸蛋白酶活性的方式调控增殖与存活，发挥抑癌作用。