食蟹猴CD3E & CD3D蛋白, His Tag&Flag Tag

Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)

分子别名（Synonym）

CD3E & CD3D,CD3 delta & CD3 epsilon

表达区间及表达系统（Source）

Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-C52W4) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5 (CD3E) & Q95LI8 (CD3D)).

Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)

蛋白结构（Molecular Characterization）

Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) and Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 15kDa, 17kDa, 27-32kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glyosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

T细胞表面糖蛋白CD3δ链和CD3ε链（分别简称CD3D与CD3E或CD3D&CD3E）属于单次跨膜I型膜蛋白。CD3δ链与CD3ε链、CD3γ链、CD3ζ链以及T细胞受体α/β和γ/δ异源二聚体共同构成T细胞受体-CD3复合物。该复合物在将抗原识别信号偶联至多条细胞内信号转导通路的过程中发挥关键作用。