人Fc gamma RIIB/C / CD32b/c蛋白, His Tag

Human Fc gamma RIIB/C (CD32b/c) Protein, His Tag (SPR & BLI & MALS verified)

分子别名（Synonym）

FCGR2B, C, CD32b, c, FcRII-b, c, Fc-gamma RII-b, c, Fc-gamma-RIIb, c, CD32, FCG2, IGFR2, CDw32

表达区间及表达系统（Source）

Human CD32b/c, His Tag (CDB-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1). In the region Ala 46 - Pro 217, the AA sequence of Fc gamma RIIB and Fc gamma RIIC are homologus.

Predicted N-terminus: Ala 46

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 20.4 kDa. The protein migrates as 28-31 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介绍

IgG Fc段受体（FcγR）是免疫球蛋白超家族成员，在免疫反应的激活或抑制中发挥作用。目前已发现三类人类Fcγ受体：RI（CD64）、RII（CD32）和RIII（CD16），这些受体可产生多种亚型。

人类FcγRII/CD32存在三种基因亚型（A、B、C），而小鼠仅存在FcγRII B（CD32B）。CD32是低亲和力的IgG受体，其亚型低亲和力免疫球蛋白γ Fc段受体II-b（FCGR2B）亦称为CD32b、FCG2或IGFR2。该受体表达于B细胞和髓系树突细胞表面。B细胞表面的CD32B受体交联作用可下调抗体生成，并在特定条件下诱导细胞凋亡；树突细胞表面的CD32B共交联（co-ligation）则抑制细胞成熟并阻断其活化。CD32B还可能成为恶性肿瘤单克隆抗体疗法的靶点。