人IFN-gamma / IFNG蛋白, premium grade

Human IFN-gamma / IFNG Protein, premium grade

分子别名（Synonym）

Interferon-gamma ,Interferon-γ

表达区间及表达系统（Source）

Human IFN-gamma, premium grade (IFG-H4211) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Gln 166 (Accession # P01579).

Predicted N-terminus: Gln 24

该产品在严格的质量控制体系下生产，涵盖无菌检测及内毒素检测等全套测试项目。其性能经严格验证，确保适用于细胞培养及临床前阶段的相关应用。

GMP-IFGH24是IFG-H4211蛋白的GMP级版本，二者性能参数无差异，可为终端用户提供从临床前研究到临床阶段的无缝衔接。

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 16.8 kDa. The protein migrates as 15 kDa, 19 kDa and 24 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method / rFC method.

宿主蛋白残留（Host Cell Protein）

<0.5 ng/µg of protein tested by ELISA.

宿主核酸残留（Host Cell DNA）

<0.02 ng/μg of protein tested by qPCR.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

γ-干扰素（IFN-γ/IFNG）是唯一的II型干扰素，为可溶性二聚化细胞因子。该分子最初被称为巨噬细胞活化因子（现指代包含IFN-γ在内的更大蛋白家族），已在多种临床适应症中应用。作为免疫应答的核心调节因子，IFN-γ通过Janus激酶（JAK）-信号转导及转录激活因子（STAT）通路传递信号，通过上调细胞周期调控、凋亡及抗原加工/呈递相关基因的转录，在激活抗病毒/抗肿瘤的固有免疫与适应性免疫中发挥广泛作用。然而，啮齿类与人类滋养层细胞对IFN-γ的应答呈现钝化现象，这源于其对IFN-γ介导的MHC II类移植抗原表达激活具有抵抗性。