人Fc gamma RIIIA / CD16a蛋白,?Avitag, His Tag

Biotinylated Human Fc gamma RIIIA / CD16a (V176) Protein, Avitag™,His Tag (HPLC & SPR & BLI verified)

分子别名（Synonym）

FCGR3A,CD16A,FCG3,FCGR3,IGFR3

表达区间及表达系统（Source）

Biotinylated Human CD16a (V176) Protein, Avitag,His Tag (CDA-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # P08637-1 (F176V)).

Predicted N-terminus: Gly 17

蛋白结构（Molecular Characterization）

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 25.5 kDa. The protein migrates as 36-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介绍 

CD16 是一种低亲和力 Fc 受体，已被鉴定为 Fc 受体 FcγRIIIa (CD16a) 和 FcγRIIIb (CD16b)。这些受体与 IgG 抗体的 Fc 段结合。CD16 由两个不同的高度同源基因以细胞类型特异性的方式编码。CD16 存在于自然杀伤细胞、中性粒细胞（多形核白细胞）、单核细胞和巨噬细胞的表面。

CD16a 抗原也称为低亲和力免疫球蛋白 γ Fc 区受体 III-A (Low affinity immunoglobulin gamma Fc region receptor III-A) 或 Fc-γ RIII-α (Fc-gamma RIII-alpha)。CD16b 是一种低亲和力、GPI 锚定的受体，表达于中性粒细胞和嗜酸性粒细胞；而 CD16a 则是一种中亲和力的多肽锚定跨膜糖蛋白，表达于自然杀伤细胞、巨噬细胞、T细胞亚群、未成熟胸腺细胞和胎盘滋养层细胞。

CD16a 参与吞噬作用、酶和炎症介质的分泌、抗体依赖性细胞介导的细胞毒性作用（ADCC）以及免疫复合物的清除。CD16a 的异常表达或突变与对反复性病毒感染的易感性、系统性红斑狼疮（SLE）以及同种免疫性新生儿中性粒细胞减少症有关。