人Glypican 3 / GPC3蛋白, His Tag, premium grade

Human Glypican 3 / GPC3 Protein, His Tag, premium grade

分子别名（Synonym）

GPC3, OCI5, Glypican-3, GTR2-2, MXR7, DGSX, SDYS , SGB, SGBS, SGBS1

表达区间及表达系统（Source）

Human Glypican 3, His Tag, premium grade (GP3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).

Predicted N-terminus: Gln 25 & Ser 359

该产品在我们严格的质量控制体系下生产，包含无菌检测、内毒素检测等全套测试。其性能经过严谨验证，并针对细胞培养用途及临床前研究阶段的其他应用场景进行了兼容性测试。当您的研究进入后期临床阶段时，我们还提供定制化GMP蛋白服务：根据您的具体需求，我们将协同开发符合细胞治疗产品制造要求的GMP级蛋白制品，确保其满足细胞生产过程中原料及辅料的使用规范。

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 24.6 kDa respectively. The protein migrates as 30 kDa, 40 kDa and 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method / rFC method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

磷脂酰肌醇蛋白聚糖-3（Glypican-3，简称GPC3）又名肠道蛋白OCI-5、GTR2-2或MXR7，属于磷脂酰肌醇蛋白聚糖家族。该蛋白在肺、肝、肾组织中高表达，能抑制二肽基肽酶IV（DPP4）的活性。GPC3可能参与调控中胚层来源组织器官的生长抑制/调节过程，并在介导胰岛素样生长因子2（IGF2）与其受体的相互作用中发挥功能调节作用。