人IL-4 R alpha / CD124蛋白, His Tag

Human IL-4 R alpha / CD124 Protein, His Tag (MALS verified)

分子别名（Synonym）

IL4R, CD124, IL4RA

表达区间及表达系统（Source）

Human IL-4 R alpha, His Tag (ILR-H5221) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # NP_000409.1).

Predicted N-terminus: Met 26

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 24.6 kDa. The protein migrates as 36-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞介素4（IL-4）是一种由活化Th2细胞与肥大细胞产生的多效细胞因子，在免疫应答中发挥核心作用。其生物学效应通过结合造血/非造血细胞表面的高亲和力受体复合物介导。在造血细胞中，IL-4信号由140kDa的IL-4Rα亚基（CD124）与70kDa的共同细胞因子受体γ链（γc, CD132）组成的高亲和力受体复合物传递。

白细胞介素4受体（IL-4R），亦称CD124、IL-4Rα或BSF受体，属于I型细胞因子受体。该受体介导Th2型免疫应答、替代性巨噬细胞活化、黏膜免疫、过敏性炎症、肿瘤进展及动脉粥样硬化等病理进程。其可溶形式（sIL-4R）可通过可变剪接或膜结合蛋白酶解产生，能竞争性阻断IL-4介导的细胞增殖及T细胞IL-5上调效应。

IL-4R还可与IL-13Rα1结合形成II型受体，该受体可同时响应IL-4与IL-13信号。经证实，IL-4R可与衔接蛋白SHC1发生相互作用。