人PSMA / FOLH1蛋白, His Tag

Human PSMA / FOLH1 Protein, His Tag (active enzyme, MALS verified)

分子别名（Synonym）

FOLH1, PSMA, GIG27, FOLH, NAALAD1, PSM, NAALADase I, GCPII, FGCP

表达区间及表达系统（Source）

Human PSMA, His Tag (PSA-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750 (Accession # Q04609-1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 81.4 kDa. The protein migrates as 95-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in MES and NaCl with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介绍

前列腺特异性膜抗原（PSMA），亦称叶酸水解酶1（FOLH1）、谷氨酸羧肽酶2（GCP2）、N-乙酰化α-连接酸性二肽酶I（NAALAD1），属于肽酶M28家族及M28B亚家族。FOLH1/PSMA在pH值高于6.5的环境中保持稳定。该蛋白是一种II型跨膜锌金属肽酶，在神经系统、前列腺、肾脏和小肠中表达量最高。

FOLH1/GCP-2以同源二聚体形式存在，每个亚基结合2个锌离子，其结构为NAALADase酶活性所必需。PSMA的催化活性主要体现为：从乙酰基-Asp-Glu或叶酰聚-γ-谷氨酸等底物中释放未取代的C端谷氨酰残基。FOLH1/GCP-2/PSMA兼具叶酸水解酶和N-乙酰化α-连接酸性二肽酶（NAALADase）活性，并对三聚α-谷氨酸肽具有作用偏好性。

GCP-2/PSMA参与前列腺肿瘤进展过程，同时表现出二肽基肽酶IV型活性。体外实验中可切割底物Gly-Pro-AMC（7-氨基-4-甲基香豆素）。