人TSLP蛋白, His Tag, premium grade

Human TSLP Protein, His Tag, premium grade

分子别名（Synonym）

TSLP

表达区间及表达系统（Source）

Human TSLP Protein, His Tag, premium grade (TSP-H52Hb) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1).

Predicted N-terminus: Tyr 29

该产品在严格的质量控制体系下生产，涵盖无菌检测及内毒素检测等全套测试。产品性能经严谨验证，确保其在细胞培养应用及临床前研究阶段其他用途的兼容性。当研究需进入临床试验阶段时，我们还提供定制化GMP蛋白服务：根据您的具体需求，我们将协同开发符合要求的GMP级产品，同时满足细胞治疗产品制造中原辅材料的使用规范。

GMP-VE5H23是VE5-H4210蛋白的GMP版本，二者具有完全一致的性能特征，可确保用户从早期临床前研究到后期临床试验阶段的无缝转化。

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.8 kDa. The protein migrates as 20-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 24 months under sterile conditions after reconstitution.

背景介绍

胸腺基质淋巴细胞生成素（TSLP）是一种上皮细胞源性细胞因子，广泛表达于上皮细胞，参与炎症性皮肤病的病理过程。人类TSLP cDNA编码的159个氨基酸前体蛋白包含28个氨基酸信号肽序列(4,5)。该因子具有诱导非缺失性中枢免疫耐受、扩大上皮细胞诱导的类别转换、引发特应性疾病及维持肠道非炎症环境等生物学功能。在响应人类TSLP的多种细胞中，CD11c+树突状细胞是特征最明确的目标细胞。