人VEGF165蛋白, His Tag

Human VEGF165 Protein, His Tag (MALS verified)

分子别名（Synonym）

RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF

表达区间及表达系统（Source）

Human VEGF165, His Tag (VE5-H5248) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 21.1 kDa. The protein migrates as 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a dimer.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

VEGF165是VEGF-A最丰富的剪接变体，由内皮细胞、巨噬细胞和T细胞等多种细胞产生。该因子参与血管生成、血管内皮细胞存活、生长、迁移及血管通透性调节。VEGF基因表达受缺氧、炎症细胞因子和癌基因诱导。VEGF165通过与硫酸乙酰肝素结合，锚定于细胞表面及细胞外基质中，并与受体酪氨酸激酶VEGFR1/VEGFR2结合。作为唯一能结合共受体神经纤毛蛋白-1（NRP-1）和神经纤毛蛋白-2（NRP-2）的剪接变体，VEGF165可增强VEGFR2信号传导。其与VEGFR1/VEGFR2的结合会诱导PI3K/AKT、p38 MAPK、FAK及桩蛋白信号通路的激活，在多种癌症的肿瘤血管生成中发挥关键作用。