人LILRB4蛋白, Fc, Avitag

Biotinylated Human LILRB4 / CD85k / ILT3 Protein, Fc,Avitag™ (MALS verified)

分子别名（Synonym）

LILRB4,ILT3,LIR5,CD85K,HM18

表达区间及表达系统（Source）

Biotinylated Human LILRB4 Protein, Fc,Avitag (LI4-H82F6) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # AAH26309.1).

Predicted N-terminus: Gln 22

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 54.4 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

白细胞免疫球蛋白样受体B亚族成员4（LILRB4），亦称CD85抗原样家族成员K（CD85K）、免疫球蛋白样转录物3（ILT-3）、白细胞免疫球蛋白样受体5（LIR-5）、单核细胞抑制性受体HM18，属于白细胞免疫球蛋白样受体（LIR）家族。该受体包含2个免疫球蛋白样C2型结构域，在单核细胞、巨噬细胞、树突细胞、肺组织、自然杀伤细胞及B细胞中表达。LILRB4/CD85K作为I类主要组织相容性复合体（MHC）抗原的受体，可识别HLA-A、HLA-B、HLA-C和HLA-G的多种等位基因，参与免疫应答的下调及免疫耐受的形成。该受体通过干扰TNFRSF5信号传导与NF-κB上调通路，抑制受体介导的细胞蛋白磷酸化及细胞内钙离子动员。