小鼠Cathepsin L / CTSL1蛋白, His Tag

Mouse Cathepsin L / CTSL1 Protein, His Tag (MALS verified)

分子别名（Synonym）

CTSL1,MEP,CATL,CTSL

表达区间及表达系统（Source）

Mouse Cathepsin L Protein, His Tag (CAL-M52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 18 - Asn 334 (Accession # P06797-1).

Predicted N-terminus: Thr 18

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. This protein contains an Activation peptide, and will be partially processed into Pro form with calculated MW of 35.8 kDa and proteolytic cleavage generate the single-chain activate form with calculated MW of 24.1 kDa and cleavaged activation peptide with calculated MW of 5 kDa under reducing (R) condition. The protein migrates as 40 kDa, 30 kDa and 10 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 25 mM Tris, 150 mM NaCl, pH7.0 with glycerol as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

组织蛋白酶L（CTSL1），亦称主要外泌蛋白（MEP），是肽酶C1家族成员，以二聚体形式存在——其重链与轻链通过二硫键连接。作为溶酶体半胱氨酸蛋白酶，CTSL1在细胞内蛋白质分解代谢中起核心作用。其作用底物包括胶原蛋白、弹性蛋白以及α-1蛋白酶抑制剂（中性粒细胞弹性蛋白酶活性的关键调控因子）。MEP参与多种病理过程：

·肌病及心肌缺血中的肌原纤维坏死

·肾小管对蛋白尿的应激反应

CTSL1对溶酶体蛋白质整体降解至关重要，其底物特异性与木瓜蛋白酶高度相似。相较于组织蛋白酶B，CTSL1对蛋白质底物具有更高水解活性，但对苄酯基-精氨酸-精氨酸-甲萘胺（Z-Arg-Arg-NHMec）作用微弱，且无肽基二肽酶活性。该酶在弱酸性环境（pH 4.5~6.5）活性最强，随pH升高稳定性显著降低。