人Claudin-6 / CLDN6蛋白, Flag,His,GFP Tag

Fluorescent Human Claudin-6 / CLDN6 Full Length Protein, Flag,His,GFP Tag (Nanodisc)

分子别名（Synonym）

Claudin-6, CLDN6

表达区间及表达系统（Source）

Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (CL6-HF2M9) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Val 220 (Accession # P56747).

Predicted N-terminus: Met

蛋白结构（Molecular Characterization）

This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag, and a GFP tag at the C-terminus.

The protein has a calculated MW of 54.0 kDa. The protein migrates as 22 kDa, 25 kDa, 51-52 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by an amphipathic scaffold coat that confers enhanced stability and a narrow particle size distribution.

The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein upon removal of the detergent.

偶联（Conjugate）

GFP

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 530 nm

纯度（Purity）

>80% as determined by SDS-PAGE.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景介绍

紧密连接是上皮或内皮细胞层中细胞间黏附的一种形式，通过在细胞周围形成连续密封结构，作为物理屏障阻止溶质和水自由通过细胞旁路。这些连接由向外侧的胞质小叶中连续网状链束组成，并与向内侧的胞外小叶中的互补沟槽嵌合。

密蛋白-6（Claudin-6，CLDN6）是密蛋白家族中的多次跨膜蛋白，由上皮细胞表达，参与组织发育和紧密连接完整性的维持。该蛋白同时是丙型肝炎病毒（HCV）进入细胞的辅助因子之一。CLDN6的甲基化修饰可能参与食管肿瘤发生，其基因位于16号染色体q28.3区，与同家族成员CLDN9相邻。