ASK1抗体;ASK1 Antibody—艾普蒂

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     Western blot analysis of ASK1 in Hela lysates using ASK1 antibody.    

  
  

The apoptosis signal-regulating kinase 1 (ASK1) antibody is a crucial tool for studying the ASK1 protein, a serine/threonine kinase central to stress-activated signaling pathways. ASK1. also known as MAP3K5. belongs to the mitogen-activated protein kinase kinase kinase (MAP3K) family. It activates downstream kinases MKK4/7 and MKK3/6. which in turn regulate JNK and p38 MAPK pathways involved in apoptosis, inflammation, and cellular responses to oxidative stress, endoplasmic reticulum (ER) stress, or cytokine stimulation. Dysregulation of ASK1 is linked to diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions.

ASK1 antibodies are widely used in research to detect ASK1 expression, phosphorylation status, and interactions in immunoblotting (Western blot), immunoprecipitation, or immunohistochemistry. Specific antibodies may target distinct regions, such as the N-terminal inhibitory domain, C-terminal kinase domain, or phosphorylated residues (e.g., Thr845 in humans), enabling analysis of ASK1 activation. For example, phosphorylated ASK1 antibodies help assess its activation during stress responses, while total ASK1 antibodies measure overall protein levels. These reagents are vital for elucidating ASK1's regulatory mechanisms, including its inhibition by thioredoxin under normal conditions or activation via TRAF2/6 in inflammatory signaling. Commercial ASK1 antibodies are often validated for species reactivity (human, mouse, rat) and application-specific performance.