4E BP3抗体;4E BP3 Antibody—艾普蒂

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     Western blot analysis of EIF4EBP3 in CHO-K1 lysates（B）and CHO-K1 transfected by EGFPEIF4EBP3 fragment（A）lysates using EIF4EBP3 antibody.    

  
  

The 4E-BP3 antibody is a crucial tool for studying the eukaryotic translation initiation factor 4E-binding protein 3 (4E-BP3), a member of the 4E-BP family (including 4E-BP1. BP2. and BP3) that regulates cap-dependent protein synthesis. 4E-BPs inhibit translation by binding to eIF4E, a key component of the eIF4F complex, thereby blocking its interaction with eIF4G and preventing ribosome recruitment. Unlike 4E-BP1 and BP2. which are regulated by mTOR-mediated phosphorylation, 4E-BP3 exhibits distinct phosphorylation patterns and expression profiles, though its precise regulatory mechanisms remain less characterized.

4E-BP3 is implicated in cellular stress responses, apoptosis, and tumor suppression. Its dysregulation has been linked to cancers, neurodegenerative disorders, and metabolic diseases. The 4E-BP3 antibody enables detection of endogenous 4E-BP3 protein levels, phosphorylation status (e.g., at Thr32 or other sites), and interactions with eIF4E in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Researchers use it to explore mTOR pathway activity, translational control in disease models, or therapeutic targeting. Specificity is critical, as cross-reactivity with homologous 4E-BP1/BP2 may occur; thus, validation using knockout controls is essential. Commercial antibodies are often raised against unique peptide regions to ensure selectivity. Its applications span cancer biology, neurobiology, and studies of cellular homeostasis.